LCMS Determination and Cytotoxicity of <i>Abrus precatorius</i> on L6 and SK-N-MC Cell Lines

Author(s):

Krishna Prabha Naduchamy and Varadarajan Parthasarathy*   Pages 1376 - 1387 ( 12 )

Abstract:

<p> Objective: The present study aimed to investigate the cytotoxic effect of various extracts derived from <i>Abrus precatorius</i> Linn. leaves on rat L6 and human SK-N-MC neuroblastoma cell lines and determine the secondary metabolites responsible for the cytotoxicity of <i>Abrus precatorius</i>. </p><p> Methods: Successive solvent extraction of <i>A. precatorius</i> leaves was carried out using the Soxhlet apparatus with solvents such as petroleum ether, chloroform, ethyl acetate, and ethanol. HPTLC fingerprinting and LC-MS studies were performed to assess the presence of secondary metabolites, such as flavonoids and phenols, in the ethyl acetate extract. Furthermore, the cytotoxic effect of extracts was tested on rat skeletal muscle cell line L6 and human neuroblastoma cell line SK-N-MC using MTT assay. </p><p> Results: The total phenolic content of ethyl acetate and ethanol extracts of <i>A. precatorius</i> were 72.67 and 60.73 mg, respectively, of GAE/g dry weight of the extract. The total flavonoid content of ethyl acetate and ethanol extract of <i>A. precatorius</i> were 107.33 and 40.66 mg of Quercetin equivalents/g dry weight of the extract. LCMS analysis demonstrated that the flavonoids in specific Naringenin, Diosmetin, Glycitin, and Genistein might play a prominent role in the cytotoxicity of <i>A. precatorius</i>. The cytotoxicity study revealed that the extracts of <i>A. precatorius</i> were non-toxic to rat L6 myotubes, and the IC₅₀ values of the various extracts, such as APPE, APCH, APEA, and APET, were >100 μg/ml. The extracts exhibited cytotoxic activity against human neuroblastoma SK-N-MC cells, and the IC₅₀ values of APPE, APCH, APEA, APET, and the standard drug “Cisplatin” were >100, >100, 64.88, >100, and 3.72 μg/ml, respectively. </p><p> Conclusion: It was concluded from the study that the extracts of <i>Abrus precatorius</i> were cytotoxic to neuroblastoma cell lines but non-toxic to normal cell lines. HPTLC and LC-MS studies confirmed that flavonoids in the ethyl acetate extract could be responsible for the biological activity.</p>

Keywords:

<i>Abrus precatorius</i>, cytotoxicity, flavonoids, HPTLC-densitometry, LC-MS, L6 myotubes, SK-N-MC.

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